Results
| PMID | 12095505 |
| Gene Name | THBS1 |
| Condition | Endometriosis |
| Association |
Associated |
| Sex | Female |
| Associated genes | VEGF, TSP-1 |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2002 Jul;78(1):148-53. Tan, Xian-Jie| Lang, Jing-He| Liu, Dong-Yuan| Shen, Keng| Leng, Jin-Hua| Zhu, Lan Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. tanxj@csc.pumch.ac.cn OBJECTIVE: To investigate the expression of vascular endothelial growth factor (VEGF) mRNA and thrombospondin-1 (TSP-1) mRNA in endometriosis. DESIGN: Molecular studies in human tissue. SETTING: Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, P. R. China. PATIENT(S): Patients undergoing laparoscopy for infertility or other benign gynecologic conditions. INTERVENTION(S): Biopsies were taken from endometriotic lesions (red peritoneal lesion, ovarian endometrioma, and unterosacral ligament nudule) and eutopic endometrium during laparoscopy. MAIN OUTCOME MEASURE(S): mRNA expression from endometriotic lesion and eutopic endometrium was analyzed by reverse transcriptase polymerase chain reaction (PCR) and Northern blotting. RESULT(S): Among the endometriotic lesions, red peritoneal lesions expressed higher levels of VEGF mRNA and lower levels of TSP-1 mRNA, whereas ovarian endometrioma expressed lower levels of VEGF mRNA and higher levels of TSP-1 mRNA. Eutopic endometrium of women with endometriosis had higher expression levels of VEGF mRNA and lower expression levels of TSP-1 mRNA than that of women without endometriosis. CONCLUSION(S): The expression of VEGF and TSP-1 in endometriotic lesions appears to be associated with the extent of their neovascularization. The imbalance in expression of VEGF and TSP-1 in the endometrium may play a role in the development of endometriosis. Mesh Terms: Adult| Blotting, Northern| Endometriosis/*metabolism| Endometrium/metabolism| Endothelial Growth Factors/*genetics| Female| Humans| Lymphokines/*genetics| RNA, Messenger/metabolism| Reference Values| Reverse Transcriptase Polymerase Chain Reacti |
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